This section is intended to provide a readily accessible list of validated non-animal toxicity test methods and should be considered as a resource for those looking for a validated non-animal alternative method/protocol.

The test methods listed in this section have been judged to be scientifically valid by the European Centre for the Validation of Alternative Methods (ECVAM), the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), or the Organisation for Economic Co-operation and Development (OECD) or have been accepted for regulatory use without formal validation.

Although the OECD encourages the validation of test methods prior to their adoption as Test Guidelines (TGs), validation is not a prerequisite for their adoption. OECD TGs are generally accepted worldwide as relevant to evaluating a human health toxicity endpoint. Accordingly, we list the in vitro test methods incorporated into TGs, even though they may not have been formally validated.

A comprehensive table of validated and/or regulatory accepted alternative test methods (in vivo and in vitro) is also provided in Validated/Accepted Alternative Methods.